Friday, July 3, 2009

Chemical Composition, Iron Bioavailability, and Antioxidant Activity of Kappaphycus alvarezzi

Kappaphycus alvarezzi, an edible seaweed from the west coast of India, was analyzed for its chemical composition. It was found that K. alvarezzi is rich in protein (16.24% w/w) and contains a high amount of fiber (29.40% w/w) and carbohydrates (27.4% w/w). K. alvarezzi showed vitamin A activity of 865 μg retinal equivalents/100 g of sample. It contained a higher quantity of unsaturated fatty acids (44.50% of the total), in which relative percentage of oleic acid was 11%, cis-heptadecanoic acid 13.50%, and linoleic acid 2.3% and 37.0% of saturated fatty acids (mainly heptadecanoic acid). K. alvarezzi was also found to be good source of minerals, viz 0.16% of calcium, 0.033% of iron, and 0.016% of zinc, which are essential for various vital biological activities. Bioavailability of iron by in vitro methods showed a higher efficiency in intestinal conditions than in stomach conditions. Ascorbic acid influenced higher bioavailability of iron. Successive extracts of n-hexane, acetone, ethyl acetate, ethanol, and direct extractables of chloroform/methanol (1:1 and 2:1) were screened for antioxidant activity using a β-carotene linoleic acid model system (B-CLAMS), DPPH (α,α-diphenyl-β-picrylhydrazyl) model system and hydroxyl radical scavenging activity. The chloroform/methanol (2:1) extract has shown 82.5% scavenging activity at 1000 ppm. Acetone fraction extracts at the 1000 ppm level showed 63.31% antioxidant activity in β-carotene linoleic acid system. The acetone extract showed 46.04% scavenging activity at 1000 ppm concentration. In the case of hydroxyl radical scavenging activity, all the extracts showed better activity at the concentrations of 25 and 50 ppm, where at the 50 ppm level ethyl acetate extract showed 76.0%, acetone 75.12%, and hexane 71.15% activity, respectively. Results of this study suggest the utility of K. alvarezzi (Eucheuma) for various nutritional products, including antioxidant for use as health food or nutraceutical supplement.

SOURCE: http://pubs.acs.org/doi/abs/10.1021/jf0493627

Thursday, July 2, 2009

Seaweed Tissue Culture

im on the stage of preparing for my Thesis proposal.
The tissue culture of the seaweed Kappaphycus alvarezii is what i'm planning to work on.
sounds interesting on my part as an aquaculturist and i have alreading a background on the laboratory culture techniques.
THanks to the Algal Production Laboratory headed by Dr. Anicia Q. Hurtado for the 30-day training on seaweed tissue culture.

My problems now would be, source of strains, chemicals (fertilizers, plant growth regulators, laboratory equipments).
In fairness, kapuy pangita..

May God help me find all these stuffs.

Life has its own meaning..

L-I-F-E..
i could hardly believe it..
daghan na au na-change s aq lyf now.
I've been doing a lot of mess.
What's going on?
Psychotic naman cguroh q ani..huhuhu.. ayaw unta

What brought about these changes in me?
Cnu?, at anoh? ung nag-trigger??
I couldnt think of anything or someone.
What i have in mind as of this moment...???
a complete make-over.. a complete change-not abruptly but gradually.
i could foment change..

Wednesday, June 17, 2009

Seaweed Tissue culture protocol

hello.

i wanna ask for some help.

to those who are familiar with the the Tissue Culture Protocol of Kappaphycus alvarezzi
(this is a variety of seaweed) kindly e-mail me at shuqigrazie_22@yahoo.com or shuqigrazie_22@hotmail.com

your help would be much appreciated as this will help much in my thesis proposal and eventually my thesis.

Thanks you very much.
God speed

2009..1st Semester Publication

contributions for "The Mast Chronicle" school year 2009-2010 1st semester publication are now accepted.

Kindly submit your articles to any of the Editorial Board or e-mail @ shuqigrazie_22@yahoo.com (the editor-in-chief)
Only bonafide students of ZSCMST are allowed to submit articles.

Outsiders are allowed to give suggestions and comments.

Thanks a lot.

Back to school

June 15..
Back to school once again..
gGgggrrr..
headwracking exams, reports, proposals..hhhmmm??? ACTIVATED??
school sucks but i love schooling.

new born son of Zeus???hahaha
owz??? stunning aura..HERCULES kaw ba yan??
nyahahahahaha

so much for that, thesis proposal intawun, magbuhat p q..
last year of my stay in ZSCMST..
i nid to prove myself worthy to be called AQUACULTURIST..charrrr...
hehe..hope so..

Career woman na jud q ani someday..
Hope God will guide me s aq job hunting in the future..

Godbless to all..
ciao

Monday, June 8, 2009

I'm back to blogging

hey guys..after more than a month of roaming around different Philippine' places..ha'ay i'm back to blogging. I kinda missed doing this stuff..
this refreshes my soul..char..hehe. yeah it's for real.. I feel like being freed from the bondages of the society around me.

A striking thought came to awaken my senses and psyche.."Leadership".. so hard to define.. very difficult to do.. How am i suppose to be a leader??? I really don't
know when and how to start.. MYSELF???? I guess so. It feels so hard to conquer my own self.. but this is where i think i'm gonna start to influence others and eventually become a true LEADER with a sense of responsibility towards my constituents.

The Student Leaders' Summit in Camiguin helped me to discover, dig deeper and bloom as a student LEADER.. I am born and made to be a LEADER... Thanks for the insights shared during the summit.

It's already late.. I need to work on my report for tomorrow's meeting.. Bon jour..
au revoir..:-)